“School of Nano-Sciences”
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| Paper IPM / Nano-Sciences / 15045 |
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Quantitative three dimensional imaging of living cells provide important information about the cell morphology and its time variation. Off-axis, digital holographic interference microscopy is an ideal tool for 3D imaging, parameter extraction and classification of living cells. Two-beam digital holographic microscopes, which are usually employed, provides high quality 3D images of micro-objects, albeit with lower temporal stability. Common-path digital holographic geometries, in which the reference beam is derived from the object beam provides higher temporal stability along with, high quality 3D images. Selfreferencing geometry is the simplest of the
common-path technique, in which a portion of the object beam itself act as the reference, leading to compact setups,using few optical elements. But it has reduced field of view and also the reference may contain object information. Here we describe the development of a common-path digital holographic microscope, employing a shearing plate and converting one of the beams into a separate reference by employing a pin-hole. The setup is as compact as self referencing geometry, while providing field of view as wide as that of a two-beam microscope. The microscope is tested by imaging and quantifying the morphology and dynamics of human erythrocytes.
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